Figure 7: Compensatory mutations within sections 3 and 4 abolish the exon skipping function of the wild-type block 6 repeats.
From: Regulation of human telomerase splicing by RNA:RNA pairing
The splicing of minigenes containing wild-type or mutant repeats, with or without replacement of sections containing compensatory mutations, are shown together with the quantification of the full-length and minus beta isoforms. The ability of the wild-type block 6 repeats to promote minus beta splicing is abolished when compensatory mutations in both sections 3 and 4 are introduced to the minigene. Data are from Hela FRT clone 8 cells stably transfected with the minigenes (n=3). Error bars represent mean±standard deviation. The results are replicated in three separate transfections in two Hela FRT clones. Images of the full-length gels are provided in Supplementary Figs 1 and 2.
