Figure 1: IRAK1 and TRAF6 are the targets of EV71-induced miR-146a. | Nature Communications

Figure 1: IRAK1 and TRAF6 are the targets of EV71-induced miR-146a.

From: Inhibition of miR-146a prevents enterovirus-induced death by restoring the production of type I interferon

Figure 1

(a) EV71 infection suppressed IRAK1 and TRAF6 expressions in protein level. (b,c) miR-146a induction and IRAK1 and TRAF6 suppressions were dose dependent. *P-value <0.05, Student’s t-test, as compared with mock infection. (d) The effect of miR-146a on the luciferase reporter vectors harbouring wild-type or mutant 3′UTRs (n=4). *P-value <0.05, Student’s t-test, as compared with wild-type 3′UTR. (e) EV71 suppressed the expressions of V5-IRAK1 and V5-TRAF6 with wild-type 3′UTRs. (f) The effect of ectopic miR-146a on endogenous expression of IRAK1 and TRAF6. RD cells were transfected with the miR-146a overexpressing vector or negative control and assayed for IRAK1 and TRAF6, and miR-146a by western blot and real-time RT–PCR (n=3), respectively. MI, mock infection; MT, mock transfection; NC, negative control. All data presented are mean±s.d.

Back to article page