Figure 4: IRAK1 and TRAF6 are the targets of mus miR-146a. | Nature Communications

Figure 4: IRAK1 and TRAF6 are the targets of mus miR-146a.

From: Inhibition of miR-146a prevents enterovirus-induced death by restoring the production of type I interferon

Figure 4

(a) miR-146a was induced in mEV71 infection. MEF cells were infected with mEV71, followed by quantification of miR-146a using real-time RT–PCR (n=3). MI, mock infection. * P-value <0.05 as compared with mock infection. (b) mEV71 infection suppressed IRAK1 and TRAF6 expressions in protein level in MEF cells. (c) Predicted mus miR-146a BSs within mus IRAK1 and TRAF6 3′UTRs (the first nucleotide following the stop codon was designated as +1). (d) The effect of the ectopic miR-146a on the endogenous IRAK1 and TRAF6. MEF cells were transfected with the miR-146a overexpressing vector or negative control and assayed for IRAK1 and TRAF6 and for miR-146a by western blot and real-time RT–PCR, respectively (n=3). MT, mock transfection; NC, negative control. (e) AntagomiR-146a restored mEV71-induced suppression of TRAF6 and IRAK1 in MEF cells. MEF cells were transfected with antagomiR-146a or antagomiR-NC followed by mEV71 infection. All data presented are mean±s.d. and all P-values are calculated by Student’s t-test.

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