Figure 5: ATM promotes the maintenance of genome integrity upon induction of blocked DSBs.
From: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
(a) Micronuclei were scored in the indicated binucleated transformed MEFs following acute treatment with 2.5 μM etoposide. Histogram bars (left) represent the average±s.e.m. of n≥600 cells from three independent experiments. Statistical significance by one-way ANOVA with Bonferroni post-test is indicated (*P≤0.05; **P≤0.01; ***P≤0.001). Representative image (right) of a treated Tpd2−/−Atm−/− cell containing multiple micronuclei is shown. Scale bar, 7.5 μm. (b) Chromosomal aberrations (both break and exchange type) were scored following acute treatment with 2.5 μM etoposide. Plot (left) shows the number of aberrations per 100 chromosomes from individual metaphase spreads obtained in at least 6 independent experiments. Average±s.e.m. and statistical significance by Kruskal-Wallis test with Dunn’s post-test is indicated (*P≤0.05; **P≤0.01; ***P≤0.001). Representative image (right) of a Tpd2−/− Atm−/− metaphase containing multiple chromosomal aberrations is shown. Scale bar, 10 μm.
