Figure 6: ATM facilitates repair of biotin-blocked DSBs.
From: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
(a) Scheme of the modified pEGFP-Pem1 system harbouring 5′-phosphate or 5′-biotin ends (left) and the expected repair product (right). (b) HEK293T cells were transfected in the presence or absence of 10 μM ATM inhibitor (KU55933) with the substrates described above, and analysed by FACS for GFP-positive cells (left) and average GFP intensity (right). In both cases, data are relative to transfection with a control pEGFP-Pem1 circular plasmid. Average±s.e.m. of four independent experiments and statistical significance by two-way ANOVA with Bonferroni post-test are shown (*P≤0.05; **P≤0.01; ***P≤0.001). (c) Percentage of plasmid-repair events not associated with detectable sequence loss. Statistical significance by Chi Square test is shown (*P≤0.05; **P≤0.01; ***P≤0.001). (d) Deletion size in plasmid repair events. Substrates as described above. Average±s.e.m. and statistical significance by Kruskal-Wallis test with Dunn’s post-test is indicated.
