Figure 2: Expression analysis of GlycineSHAT1-5.
From: Pod shattering resistance associated with domestication is mediated by a NAC gene in soybean
(a) GmSHAT1-5 protein localized in developing FCC of stage-5 fruit (13 DPA) (red arrow). (b) GmSHAT1-5 protein intensely accumulated in FCC of stage-6 fruit (18 DPA) with signal stronger in the upper part. (c) Negative control with empty primary antibody generating no signal. (d) Expression analysis of all orthologues in the FCC. (e) Expression analysis of all orthologues in the AL. Note: GmSHAT1-5 expressed at 15.02-fold the level of GsSHAT1-5 in FCC, while other genes expressed in HEINONG44 0.6~4-fold over their counterparts in ZYD00755. Error bars in (d,e) indicated s.d. of four biological replicates. Scale bars in (a–c), 70 μm.
