Figure 3: Functional analysis of GlycineSHAT1-5 in Arabidopsis nst1-1;nst3-1 mutant.
From: Pod shattering resistance associated with domestication is mediated by a NAC gene in soybean
(a) Expression of GmSHAT1-5/GsSHAT1-5 under control of AtSND1 promoter effectively restores mutants to erect stem. (b) Anatomical analysis reveals that the specific expression of GmSHAT1-5/GsSHAT1-5 under control of AtSND1 promoter can activate secondary cell wall deposition in the stem interfascicular fibres (red triangles) of nst1-1;nst3-1 mutant. (c) Expression of GmSHAT1-5/GsSHAT1-5 under control of AtNST1 promoter effectively restores the indehiscent fruit phenotype of nst1-1;nst3-1 mutant (red triangles). (d) Anatomical analysis reveals that the specific expression of GmSHAT1-5/GsSHAT1-5 under control of AtNST1 promoter rescues the indehiscent fruit by activating secondary wall thickening in the valve margins and endocarps (red triangles) of nst1-1;nst3-1 mutant. Scale bars in (a), 10 cm; (b), 70 μm; (c), 500 mm; (d), 40 μm.
