Figure 7: Functional analysis of GARP-biding site in Arabidopsis protoplast.
From: Pod shattering resistance associated with domestication is mediated by a NAC gene in soybean
(a) The 20 bp deletion in the upstream regulatory region of GmSHAT1-5 in HEINONG44. The GARP-biding site is underlined with the core-biding site indicated by red letters. (b) Diagrams of the reporter constructs used for transient expression analysis. WT, tetramer of the wild type 20 bp InDel sequence; MU, tetramer of mutant sequence (the mutated core GARP-binding site was indicated by lower case); DE, tetramer of deletion sequence with core GARP-binding site deleted; mpCaMV, minimal promoter of the CaMV 35S promoter. (c) Transient expression analysis of GARP-binding site in Arabidopsis protoplast. Error bars in (c) indicate s.d. of three biological replicates.
