Figure 1: Production of moth pheromones in N. benthamiana leaf cells by transient expression of introduced genes. | Nature Communications

Figure 1: Production of moth pheromones in N. benthamiana leaf cells by transient expression of introduced genes.

From: A plant factory for moth pheromone production

Figure 1

Introduced enzymes and pathways are depicted in magenta. 16C pheromones are produced from preexisting cytosolic pool of 16:CoA whereas 14C pheromones are produced from a cytosolic 14:CoA pool created by introduction of a Cuphea palustris thioesterase intersecting chloroplast de novo acyl synthesis at 14C level. The genes for the enzymes involved in the pheromone production in this study were cloned from various moth and plant species: CpFATB2, Cuphea palustris 14:ACP thioesterase; AveΔ11, Argyrotaenia velutinana desaturase; CroΔ11, Choristoneura rosaceana desaturase; CpaE11, Choristoneura parallela desaturase; OnuΔ11, Ostrinia nubilalis desaturase; AtrΔ11, Amyelois transitella desaturase, HarFAR, Helicoverpa armigera acyl reductase; YroFAR, Yponomeuta rorellus acyl reductase, OnuFAR_E/Z, Ostrinia nubilalis acyl reductase, EaDAcT, Euonymus alatus acetyltransferase. Acyl intermediates in the pathway (also throughout the article) are given as short forms, for instance, E/Z11-14:CoA refers to the fatty-acyl coenzyme A with a chain length of 14-carbon atoms and a double bond at Δ11 position in ‘E’ or ‘Z’ configuration. ACP, acyl carrier protein; FFA, free fatty acid; OH, fatty alcohol; OAc, acetate.

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