Table 1 Characterization of candidate genes for insect pheromone production in plants.

From: A plant factory for moth pheromone production

Genes

Substrate

Product

Conversion rate (%) *

CpFATB2

14:ACP

14:acyl

High†

AtrΔ11

16:CoA

Z11-16:acyl

49.5±2.8‡

AveΔ11

14:CoA

E11-14:acyl

9.3±0.5‡

  

Z11-14:acyl

33.0±1.9‡

CroΔ11

14:CoA

E11-14:acyl

16.5±0.2‡

  

Z11-14:acyl

36.9±0.7‡

CpaE11

14:CoA

E11-14:acyl

22.7±0.8‡

OnuΔ11

14:CoA

E11-14:acyl

ND§

  

Z11-14:acyl

ND§

HarFAR

14:CoA

14:OH

4.6±1.0||

 

E11-14:CoA

E11-14:OH

11.3±2.3

 

Z11-14:CoA

Z11-14:OH

7.8±1.7#

 

16:CoA

16:OH

0.4±0.1**

 

Z11-16:CoA

Z11-16:OH

1.1±0.3††

HarFAR_KKYR

14:CoA

14:OH

14.5±2.8||

 

E11-14:CoA

E11-14:OH

28.2±5.8

 

Z11-14:CoA

Z11-14:OH

19.5±3.9#

 

16:CoA

16:OH

1.0±0.1**

 

Z11-16:CoA

Z11-16:OH

5.2±1.3††

YroFAR

14:CoA

14:OH

0.9±0.5‡

 

E11-14:CoA

E11-14:OH

2.1±1.6‡

 

Z11-14:CoA

Z11-14:OH

0.4±0.2‡

 

16:CoA

16:OH

0.3±0.1‡

 

Z11-16:CoA

Z11-16:OH

0.1±0.0‡

OnuFAR_Z

Z11-14:CoA

Z11-14:OH

<0.1±0.0‡

OnuFAR_E

E11-14:CoA

E11-14:OH

ND§

EaDAcT

14:OH

14:OAc

7.0±2.7‡

 

E11-14:OH

E11-14:OAc

17.9±5.5‡

 

Z11-14:OH

Z11-14:OAc

13.0±1.6‡

 

16:OH

16:OAc

6.0±1.4‡

 

Z11-16:OH

Z11-16:OAc

7.6±1.7‡

  1. The attachment of KKYR retention signal after the HarFAR resulted in better conversion rate when compared with the wild-type one, tested by t-test.
  2. *conversion rate=(product/(product+remaining substrate))*100%±s.e.
  3. †Amount of 14:acyl (produced from naturally occurring 14:ACP) was ca. 100-fold higher in treated compared with control plants.
  4. ‡±s.e. n=3.
  5. §ND, not detectable.
  6. ||P=0.029, n=4, two sample t-tests.
  7. P=0.042, n=4, two sample t-tests.
  8. #P=0.039, n=4, two sample t-tests.
  9. **P=0.005, n=4, two sample t-tests.
  10. ††P=0.037, n=4, two sample t-tests.
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