Figure 2: Paternal MHC regulates uNK responsiveness.
From: MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling
(a) Gating strategy for Ly49C−I−Ly49A+ uNK (gd10.5) after exclusion of dead cells and CD3+ T cells. FMO, fluorescence minus one. (b,c) Frequency of IFN-γ+ NK cells (Ly49C−I−as gated in (a)) in response to IL-12 and IL-15 among Ly49A+ (b) or Ly49D+ (c). (d) Responsiveness of bulk NK cells and those expressing Ly49A or Ly49G2 (irrespective of expression of other Ly49) from B6 females mated with D8 males normalized to the responsiveness of uNK cells from B6 females mated with B6 males. (e) Responsiveness of uNK subsets with or without receptors recognizing H-2Dd, n=5–8 mice per group. Means±s.e.m. P-values from unpaired Student’s t-tests, *P<0.05.
