Figure 5: NR4A1 promotes TGF-β-induced EMT and is associated with bad prognosis in breast cancer patients.
From: Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-β signalling
(a) Light image of HaCaT cells stably expressed with empty vector (Con.ve) or NR4A1. (b,c) Immunofluorescence of HaCaT cells stably expressing NR4A1 (b) or with knockdown of NR4A1 (c) and treated with or without TGF-β (5 ng ml−1) for 48 h. Scale bar, 20 μm. (d) Immunoblot analysis of MCF10A cells stably expressing or with knockdown of NR4A1 and treated with TGF-β (5 ng ml−1) and SB431542 (10 μM) for 48 h. Actin immunoblot has been used as a loading control. (e) Scatter plot showing the positive correlation between NR4A1 and Slug/Vimentin/Fibronectin expression in the NKI295 patients’ clinical data set (n=295). Pearson’s coefficient tests were performed to assess statistical significance.
