Figure 3: Trk is cleaved multiple times. | Nature Communications

Figure 3: Trk is cleaved multiple times.

From: Trunk cleavage is essential for Drosophila terminal patterning and can occur independently of Torso-like

Figure 3

(a) Immunoblot without prior immunoprecipitation of protein extracted from embryos expressing HA:Trk:Myc. Only full-length Trk (FL) is detected using anti-Myc. (b) Immunoblot of protein extracts immunoprecipitated (using anti-Myc) from embryos expressing Trk constructs and blotted with anti-Myc. HA:Trk:Myc extracts contain a predominant Trk species (FL=full length) and at least six C-terminal Trk fragments (arrows) not observed in untagged Trk extracts (nos>Trk). Two independent TrkK75A,R76A mutants lack fragments 1 and 2. (c) Immunoblot of protein extracts immunoprecipitated (using anti-Myc or anti-HA) from embryos expressing HA:Trk:Myc and blotted with anti-HA. Note that full-length (FL) Trk protein immunoprecipitated with anti-Myc can be detected with anti-HA. No additional N-terminal Trk fragments are observed in extracts immunoprecipitated with anti-HA. * indicates immunoglobulin bands. Images are representative of at least two replicate immunoblots.

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