Figure 5: 1H NMR studies of (R)-2HG oxidation mediated by H2O2.
From: Non-enzymatic chemistry enables 2-hydroxyglutarate-mediated activation of 2-oxoglutarate oxygenases
Reaction of (R)-2HG with H2O2 in a 90% water 10% D2O mixture ((a) 5 mM (R)-2HG, (b) 5 mM (R)-2HG, 50 μM Fe(II); (c) 5 mM (R)-2HG, 10 mM L-ascorbate; (d) 5 mM (R)-2HG, 5 mM H2O2, (e) 5 mM (R)-2HG, 50 μM Fe(II), 5 mM H2O2; (f) 5 mM (R)-2HG, 10 mM L-ascorbate, 5 mM H2O2; (g) 5 mM (R)-2HG, 50 μM Fe(II), 10 mM L-ascorbate; (h) 5 mM (R)-2HG, 50 μM Fe(II), 10 mM L-ascorbate, 5 mM H2O2). 2HG, 2-hydroxyglutarate; 2OG, 2-oxoglutarate; SA, succinic acid; SSA, succinic semialdehyde. Addition of both Fe(II)/L-ascorbate/H2O2 leads to impaired 2OG and SSA formation, likely due to competition between different oxidative pathways and/or further oxidation of 2OG and SSA.
