Figure 6: Carnitine formation catalysed by BBOX1 in the presence of 2HG.
From: Non-enzymatic chemistry enables 2-hydroxyglutarate-mediated activation of 2-oxoglutarate oxygenases
(a) Standards of (S)-2HG (2-hydroxyglutarate) and (e) (R)-2HG; (b) (S)-2HG or (f) (R)-2HG incubated with the assay mixture for 24 h prior to analysis; (c) (S)-2HG or (g) (R)-2HG incubated in the assay mixture with BBOX1 for 24 h prior to analysis; (d) (S)-2HG or (h) (R)-2HG incubated in the assay mixture for 24 h, then treated with BBOX1 and Fe(II) (1:1) and incubated for a further 2 h prior to analysis. The standard assay mixture contained 100 μM GBB (γ-butyrobetaine), 4 mM L-ascorbate, 50 μM Fe(II), 80 mM KCl in 50 mM Tris-d11 pH 7.5 containing 10% D2O. BBOX1 concentration was 100 nM. (S)-2HG and (R)-2HG were used at a final concentration of 5 mM.
