Figure 10: The Sema3A-enhanced dendritic GluA2 regulates dendritic patterning both in vitro and in vivo. | Nature Communications

Figure 10: The Sema3A-enhanced dendritic GluA2 regulates dendritic patterning both in vitro and in vivo.

From: Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors

Figure 10

(a) Knockdown of GluA2-suppressed Sema3A-induced dendritic branching of cultured hippocampal neurons. This knockdown effect was rescued by expressing a EGFP-GluA2res. Arrowheads indicate MAP2-positive dendritic branching point. Normalized mean number of dendritic branching points per neuron is shown in b (n=27–41 neurons from more than three independent cultures). (c) Compared with empty-vector-electroporated neurons, apical dendrites branched shortly after they enter the sr region in the PlexA1-IPT overexpressed neurons (arrowheads). The relative position of the first branch point of apical dendrite is shown in d (n=108–109 neurons from three individual brains). The relative length of the sr was set to 1 (position 0 is the border between sp and sr, position 1 the border between sr and slm). Scale bars in a, 25 μm; c, 50 μm. *P<0.05, **P<0.01 by ANOVA compared with vehicle control (b) or t-test compared with without PlexA1-IPT (empty vector, d). sp, stratum pyramidale; sr, stratum radiatum; slm, stratum lacunosum-moleculare. Data are presented as mean±s.e.m.

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