Figure 2: Sema3A induces dendritic localization of GluA2 in a GRIP1- and KIF5-dependent manner. | Nature Communications

Figure 2: Sema3A induces dendritic localization of GluA2 in a GRIP1- and KIF5-dependent manner.

From: Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors

Figure 2

(a) Knockdown of GRIP1 attenuated Sema3A-enhanced immunostaining of GluA2 in dendrites. This attenuation was rescued by expressing a siRNA-resistant form of EGFP-GRIP1 (GRIP1res). Normalized mean immunostaining intensity of dendrite per neuron is shown in b (n=36–50 neurons from more than three independent cultures). (c) Overexpression of KIF5 DN mutant (KHCCBD) attenuated Sema3A-enhanced immunostaining of GluA2 in dendrites. Normalized mean immunostaining intensity of dendrite per neuron is shown in d (n=29–30 neurons from three independent cultures). Magnified images of boxed areas in upper panels are shown in lower panels. Arrowheads indicate axon. Scale bars, 50 μm (upper panel);10 μm (lower panel). *P<0.01 by ANOVA compared with vehicle control. Data are presented as mean±s.e.m.

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