Figure 7: PlexA4 interacts with GluA2 through its IPT domain. | Nature Communications

Figure 7: PlexA4 interacts with GluA2 through its IPT domain.

From: Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors

Figure 7

(a) Structure of PlexAs and schematic representation of constructed PlexAs mutant. PlexAs contain signal peptides, sema domain, three PSI (domains found in plexin, semaphorin and integrin) domains, three IPT (immunoglobulin-like plexin transcription factor) domains, transmembrane domain and cytosolic domain. Abbreviations are as follows: Full, full length; DN, dominant negative; IPT, three IPT; Δect, lacking the entire ectodomain; ΔIPT, lacking three IPT ectodomain. (b) EGFP-tagged GluA2 interacted with full-length or IPT domain of PlexA4-Myc. (c) EGFP-tagged GluA2 weakly interacted with ΔIPT mutant of PlexA4-Myc. (d) Flag-tagged NRP1 interacted with full length, but not IPT domain, of PlexA4-Myc. (e) GluA2 and PlexA4 were co-immunoprecipitated from rat brain lysates. (f) A Sema3A-induced increase in the number of PlexA4–GluA2 interacting signals detected by in situ PLA technology in cell body followed by an increase in dendrites. The mean number of PlexA4-GluA2 interacting signals per 10 μm2 (soma) or 10 μm (dendrite) is shown in g (n=45 neurons from three independent cultures). Scale bar, 20 μm. *P<0.01 by ANOVA compared with 0 min. Data are presented as mean±s.e.m. Full scan of western blot of b–e are shown in Supplementary Fig. 7a–g.

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