Figure 8: Restoration of hepatic PRSS8 levels improves insulin resistance in db/db mice. | Nature Communications

Figure 8: Restoration of hepatic PRSS8 levels improves insulin resistance in db/db mice.

From: The serine protease prostasin regulates hepatic insulin sensitivity by modulating TLR4 signalling

Figure 8

(a) The levels of PRSS8 and TLR4 in livers from db/m and db/db mice under fasting and refeeding conditions were analysed by western blotting. Representative western blot images are shown (n=4 mice per group). (b) Blood glucose levels during a GTT and PTT, and the AUC for the PTT in db/m and db/db mice infected with Ad-LacZ or Ad-hPRSS8 (n=10 mice per group). Values are shown as the mean±s.d.; *P<0.05, **P<0.01 and ***P<0.001 for db/db-Ad-hPRSS8 versus db/db-Ad-LacZ (two-way ANOVA). †††P<0.001 versus db/m-Ad-LacZ (one-way ANOVA). (c) Western blotting for insulin-stimulated Akt phosphorylation and the level of TLR4 and PRSS8 in livers from db/m and db/db mice infected with Ad-LacZ or Ad-hPRSS8. Representative western blot images are shown (n=4 mice per group). (d) Blood glucose levels during a GTT and PTT, and the AUC for the PTT in db/m and db/db mice transfected with Control or TLR4 siRNA (n=8 mice per group). Values are shown as the mean±s.d.; *P<0.05, **P<0.01, and ***P<0.001 for db/db-TLR4 KD versus db/db-Control KD (two-way ANOVA). †††P<0.001 versus db/m-Control KD (one-way ANOVA). (e) Western blotting for insulin-stimulated Akt phosphorylation and the TLR4 protein level in livers from db/m and db/db mice transfected with Control or TLR4 siRNA. Representative western blot images are shown (n=4 mice per group).

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