Figure 2: Accumulation of p62 LC3 and Ub+ protein in Hace1−/− sTAC hearts.
From: HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress
Representative immunohistochemical staining for (a) ubiquitinated protein (brown); (b) p62 (brown) and (c) GFP-LC3 (brown) in paraffin sections of heart revealing increased accumulation of p62, ubiquitinated protein and LC3 in Hace1−/− sTAC hearts. Hearts from three mice in each group were analysed (scale bars, 10 μm). Representative immunoblot and quantification for (d,e) Ub-conjugated proteins, (d,f) p62 and (g,h) LC3 in heart extracts (n=3 per group), Gapdh were used as a loading control. (i) Increased Ub proteasome activity in Hace1−/− sTAC hearts as measured by 20S proteasome activity assay (n=4–6 per group). In all panels, error bars represent s.e.m., P value between KO and WT sTAC as indicated (one-way analysis of variance).
