Figure 7: Discriminating novel subgroups within purified haematopoietic stem cells (HSCs) from young (2 months old) mice.

(a) List of the three genes candidates that resulted in population splitting. Because of the large number of single cells that were processed (on average ~2,000 single cells per gene) the separations exhibit a high HLS score with statistical significance (P<0.001). (b) List of the four gene candidates that did not split the young HSC population. (c) Signalling lymphocytic activation molecule (Slamf1) is a representative candidate gene that separates, in a bi-modal manner, the young HSC population into high and low expressers. (d) Deoxynucleotidyl transferase terminal (Dntt) on the other hand is a representative candidate gene that is differentially expressed in young and old HSCs but does not show a bimodal expression profile within the young HSC population.