Figure 2: Functional screen for proinvasion genes.
From: MTSS1 is a metastasis driver in a subset of human melanomas
(a) Left, qRT-PCR to determine mRNA levels of the indicated genes 48 h after RNA interference in human A375 melanoma cells. Relative mRNA levels are shown as percent of control-treated cells (Ct.siRNA). Error bars represent±s.d. calculated from duplicates. Right, invasive potential of human A375 melanoma cells upon RNA interference with expression of the indicated genes as determined in a Matrigel invasion assay. The assay was performed in duplicates. Each bar represents the mean of 12 random microscopic fields±s.d. Data were compared with control-treated cells (Ct.siRNA) using the two-tailed Student’s t-test, *P≤0.05, **P≤0.01, ***P≤0.001, NS: not significant. (b) MTSS1 transcript levels in human primary and metastatic melanoma samples as obtained from cDNA microarray analysis. Upper panel, primary melanoma (blue, n=31) and lower panel, metastatic melanoma tissue samples (yellow, n=52) plotted along relative MTSS1 transcript levels. Checkered bars represent tissue samples with an outlier expression profile. Arrowheads indicate tissues samples with an underlying copy number gain. (c) Left, invasive potential of human A375 melanoma cells analysed 48 h after RNA interference with control (Ct.siRNA) and additional MTSS1 siRNAs. Bars represent mean±s.d. of three replicates. Relative invasion into Matrigel compared by the two-tailed Student’s t-test, ***P≤0.001. Arrows highlight membrane pores and cells. Right, immunoblots show MTSS1 knockdown.
