Figure 2: Positional cloning of EG1.

(a) The location of EG1 on the BAC clone AP003133 on chromosome 1. (b) A schematic representation of the EG1 protein. The mutant alleles are indicated by red lines and triangles. The conserved lipase III domain (GMSMG) is indicated by a blue bar. (c) Spikelet morphology of the wild type, eg1-3, eg1-1 and F1 progeny from eg1-3 x eg1-1. Scale bars, 1 mm. (d) Sequence analysis of the EG1 gene in F1 progeny, showing mutated nucleotides in the mutant alleles. gl, glume-like; le, lemma; pa, palea; sl, sterile lemma.