Figure 3: EG1 is involved in JA production.

(a) Overexpression of EG1 in the dad1 mutant rescued anther indehiscence (insets) and silique development (arrows) phenotypes. Scale bar, 1 cm. (b) The defects in eg1-3 spikelets were rescued by expressing EG1_pro:DGL. Sky blue indicate extra glumes. Scale bars, 1 cm for the branches, 1 mm for spikelets. (c) Comparison of endogenous JA content in the wild type and eg1-3. The aerial part of 6-day-old seedlings, 2–4 mm long inflorescence and spikelets at late stage of Sp8 were used. Each data point is the average of three biological repeats, and error bars indicate s.d. Asterisks indicate significant difference between samples (Student’s t-test P-values, *P<0.05; **P<0.01). (d) Exogenous application of MeJA rescued the abnormal spikelet development phenotype in eg1-3. Sky blue indicates extra glumes in eg1-3 spikelets. Scale bar, 1 cm. (e) Quantification of wild type-like spikelets in the inflorescence after MeJA treatment in (d). Each data point is the average of 10 biological repeats, and error bars indicate s.d. Asterisks indicate significant difference between samples (Student’s t-test P-values, *P<0.05; **P<0.01). gl, glume-like; le, lemma; lo, lodicule; lol, lodicule-like; pa, palea; sl, sterile lemma; st, stamen.