Figure 8: OsMYC2 regulates the transcription of OsMADS1.

(a) Diagram of the OsMADS1 genomic region showing the five G-box motifs, G1 to G5. Black boxes are exons. (b) qChIP-PCR results showing that the promoter fragments G1 and G2 can be amplified from IP pulled down by the anti-OsMYC2 antibody. In the control, immunoglobulin G was set as 1. Each data point represents the average of three biological repeats, and error bars indicate s.d. Asterisks indicate significant difference between samples (Student’s t-test P-values, *P<0.05; **P<0.01). (c) Yeast one-hybrid analysis showing interaction between OsMYC2 and the M2 region of OsMADS1 promoter. M1, M2, M3 are shown in (a). 3-amino-1,2,4-triazole, 3-amino-1,2,4-triazole. (d) DNA-binding assays showing OsMYC2’s binding to G2 in the promoter of OsMADS1. The precipitated OsMYC2 was detected by anti-OsMYC2 antibody. Molecular weight of proteins, MBP-OsMYC2=123 kDa, MBP=51 kDa. (e) Transient dual-LUC reporter gene assays showing OsJAZ1’s ability to inhibit OsMYC2’s transcription activity on the OsMADS1 promoter. Constructs used in the transient transactivation assays are shown in the upper panel. Five biological repeats were performed for each sample, error bars indicate s.d. Asterisks indicate significant difference between samples (Student’s t-test P-values, *P<0.05; **P<0.01). There was no significant difference between OsMYC2+GFP and OsMYC2 (Student’s t-test P-values, P>0.05).