Figure 5: TLR3-mediated crypt cell death after TBI depends on p53.
From: Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome
(a) TUNEL-staining of small intestinal crypts of p53+/+ and p53−/− mice 6 h after TBI and isologous BMT. TUNEL-stained epithelium shows green fluorescence. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. Lower panel shows numbers of TUNEL-positive cells. n=3; results are means±s.e.m. *P<0.05 (unpaired two-tailed Student’s t-test). (b) Quantitative real-time PCR of mRNA encoding Bax and PUMA in the small intestines of p53+/+and p53−/− mice. n=3; results are means±s.e.m. *P<0.05; **P<0.01 (unpaired two-tailed Student’s t-test). (c) Immunohistochemical detection of p53 in the small intestinal crypts of Tlr3+/+ and Tlr3−/− mice 0 and 6 h after TBI and isologous BMT. Scale bar, 100 μm. Lower panel shows numbers of p53-positive cells. n=3; results are means±s.e.m. N.D., not detected. (d) Quantitative real-time PCR of mRNAs encoding Bax and PUMA in the small intestines of Tlr3+/+ and Tlr3−/− mice. n=3; results are means±s.e.m. *P<0.05; **P<0.01 (unpaired two-tailed Student’s t-test). (e) TUNEL-staining of the small intestinal crypts of p53+/+and p53−/− mice 6 h after poly I:C injection. TUNEL-stained epithelium shows green fluorescence. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. The right-hand panel shows numbers of TUNEL-positive cells. n=3; results are means±s.e.m. Results are representative of three independent experiments.
