Figure 2: Contribution of transplanted BM to the small intestine. | Nature Communications

Figure 2: Contribution of transplanted BM to the small intestine.

From: Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice

Figure 2

WT and PUMA KO BMT (GFP+) recipients. (a) IF demonstrating GFP+ BM engraftment in the small intestine. Scale bar, 100 μm. (b) GFP/cytokeratin-double IF identifying donor-derived intestinal epithelium. Scale bar, 100 μm. (c) GFP/CD45-double IF identifying donor-derived CD45+ cells in the crypt and villus regions. Scale bar, 50 μm. (d) GFP/CD105-double IF identifying donor-derived CD105+ cells in the crypt and villus regions. Scale bar, 50 μm. (e) GFP/α-SMA-double IF identifying donor-derived α-SMA+ cells (fibroblasts) in the crypt and villus regions. Arrowheads indicate GFP/lineage double-positive cells. Scale bar, 50 μm. Images are representative for n=3 mice per group.

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