Figure 3: Inhibition of migration due to increased acetylation of microtubules can be rescued by counterbalancing with increased contractility. | Nature Communications

Figure 3: Inhibition of migration due to increased acetylation of microtubules can be rescued by counterbalancing with increased contractility.

From: MYPT1 regulates contractility and microtubule acetylation to modulate integrin adhesions and matrix assembly

Figure 3

(a) Relative migration velocity of HFFs stably expressing GFP (control) or different tubulins as indicated. Velocities were normalized to the control. A minimum of 50 time points were used per measurement from >30 cells (n>3). (b) Relative migration velocity of TSA-treated HFFs (5 μM TSA) expressing GFP (control), GFP-tagged phospho-mimetic MLC (rMLC-DD; activated) or GFP-tagged phospho-null MLC (rMLC-AA; negative). Velocities were normalized to the control. A minimum of 40 time points were used per measurement from >20 cells (n=3). (c) Relative migration velocity of HFFs expressing Flag-tagged HDAC6 treated with 20 μM ML-7. Each velocity was normalized to the control. A minimum of 30 time points were used per measurement from >45 cells (n=3). Data are mean±s.e.m.*P<0.05. Two-way analysis of variance (ANOVA) followed by Tukey post test was used for a, and Student’s t-test was used for b and c.

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