Figure 2: JQ1 inhibits cell growth and induces apoptosis in human osteosarcoma cell lines. | Nature Communications

Figure 2: JQ1 inhibits cell growth and induces apoptosis in human osteosarcoma cell lines.

From: Selective inhibition of BET bromodomain epigenetic signalling interferes with the bone-associated tumour vicious cycle

Figure 2

(a) Chemical structure of JQ1. (b) Human osteosarcoma cell lines (U2OS, MNNG/HOS, MG63, KHOS, SJSA-1, 143B, Cal72, G292 and SAOS2) were cultured for 72 h in the presence of JQ1 at the indicated concentration, and cell growth was determined by crystal violet assay and compared with control. GI50 for JQ1 in tumour cell lines. (c) Osteosarcoma cells were treated with 1 μM JQ1 for 2 days, and then plated at clonal density for colony counts (d). (e) Osteosarcoma cells were treated with 1 μM JQ1 for 2 days, and the proportion of cells in subG1, G0–G1, S, G2–M was determined by propidium iodide staining. (f) Osteosarcoma cells were treated with 1 or 10 μM JQ1 for 2 days and apoptosis was evaluated by cleaved poly (ADP-ribose) polymerase (PARP) level by western blotting. All experiments were repeated at least twice. Error bars show s.e.m. for n=3 measurement from representative experiments.

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