Figure 1: Warping neurons using dendritic fiducial markers (a) Side projection of an image stack.
From: A genetic and computational approach to structurally classify neuronal types
Partial RGC (green) and starburst amacrine cell (red) channels merged. (b) The detected dendritic arbor (green) and starburst sublayers (meshes) of the cell in (a). The red (On, bottom) and blue (Off, top) meshes have opaque white faces. Extents of the meshes were chosen slightly differently for visualization. (c) Dendritic arbors (left) and stratification profiles (right) of two RGCs, which were imaged in two different stacks and registered to each other using the On (red) and Off (blue) starburst planes as landmarks. The stratification profile of a cell is its distribution of dendritic length across the depth of the inner plexiform layer. This one-dimensional function is useful in visualizing the cell’s laminar positioning. The green RGC is the result of a nonlinear transformation of the RGC in (a) that flattened the starburst surfaces. The black RGC was obtained by a similar procedure from another image stack. Scale bar, 40 μm.
