Figure 4: Automated clustering of RGCs into 15 types reveals submicron reproducibility of inner plexiform layer depth within each type.
From: A genetic and computational approach to structurally classify neuronal types
Normalized stratification profiles of genetically defined cells sorted by their structural clusters (a) and at-large cells forming new structural clusters (b). Each curve corresponds to a single neuron. The dendritic length of each neuron (area under curve) is normalized to unity. Type E consisted of both W3 (dashed) and BDb cells (solid). Known functional attributes of genetically defined cells are indicated on the corresponding plots. The On and Off starburst planes are located at 0 and 12 μm, respectively. The ganglion cell layer (GCL) is located to the left and the inner nuclear layer (INL) is located to the right. (c) Stratification properties of all 15 types. Stratification distance refers to the distance of peak stratification plane to the ON starburst layer. Images of the cells are not aligned with the profiles—refer to the labels instead. Scale bars, 40 μm.
