Figure 8: The stomatin domain of STOML3 is necessary to sensitize Piezo currents in N2a cells.
From: Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch
(a) Inverted epifluorescence images of Chimera1-mGFP and Chimera2-mCherry in N2a cells (observations made in 15 cells/4 transfections and 14 cells/4 transfections respectively; scale bar, 10 μm). Both chimeras localized to a vesicle pool and the plasma membrane. (b) Schematic representation of the two chimera proteins. (c) Stimulus–response data for Chimera1-mGFP (yellow circles; 220 measurements, 15 cells) and Chimera2-mCherry (blue circles; 299 measurements, 14 cells) in N2a cells. Control N2a cell data re-plotted here for comparison (open triangles) Data were binned by stimulus magnitude and current amplitudes within each bin averaged for each cell and then between cells, presented as mean±s.e.m. To test for significance, Student's t-test was used; *P<0.05. Chimera2, containing the stomatin-domain from STOML3, sensitized mechanically mediated currents more effectively than did Chimera1.
