Figure 1: H3K9ac and PCAF involvement in the regulation of regeneration genes.
From: PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system
(a) Schematic diagram of SNA and DCA injury models used for epigenetic screens involving DNA methylation arrays and quantitative ChIP assays from L4-L6 DRG. Scale bar, 100 μm. (b) Fold change increases observed in GAP-43, Galanin and BDNF gene expression at 1, 3 and 7 days post SNA but not DCA and at 3 and 7 days for SCG-10. (c) Increased gene expression, H3K9ac, PCAF and decreased H3K9Me2 at GAP-43, Galanin and BDNF, but not SCG-10 (SCG-10 had decreased H3K9me2 enrichment to a lesser extent) promoters following 1 day post-SNA versus DCA. (d) A preconditioning lesion performed 1 week before DCA still induced 24 h later gene expression of GAP-43, Galanin and BDNF but not SCG-10. (e) This correlated with an increase in PCAF at the promoters of activated regeneration genes. Q-PCR. (b,c) N=3 per group; ChIP assays (c–e) N=6 per group, Student’s t-test, error bars, s.e. *P<0.05, **P<0.01, ***P<0.001. All experiments were performed in triplicate.
