Figure 6: ERK kinase inhibition blocks neurite outgrowth after conditioning lesion.
From: PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system
(a–c) PD98059 when applied at the nerve stump compared with Vehicle at the time of a conditioning lesion or in Sham significantly repressed neurite outgrowth 12 h later in ex vivo cultures on both laminin and myelin substrates, ICC (βIII Tubulin). Scale bars, 50 μm (a), average neurite length analysis (b) and western blot and intensity analysis showing significant reduction in pERK after PD98059 delivery (c). (b) P<0.0001, ANOVA, Bonferroni post hoc tests, **P<0.001 and ***P<0.001. (c) Student's t-test, ***P<0.001, N=3–6, performed in triplicate. Original immunoblot images are shown in Supplementary Fig. 14.
