Figure 1: Skin screen strategy and exploitation of the resource.
From: Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen
(a) Screen strategy. On receipt, tail skin was divided into three parts: one was processed for epidermal wholemount immunostaining, one for paraffin conventional histology and one for genomic DNA extraction. (b) WT epidermal wholemount image labelled for K14 (red), K15 (green) and DAPI (blue) showing hair follicle (HF), interfollicular epidermis (IFE), infundibulum (IFD), sebaceous gland (SG), bulge, scale and anagen and telogen HFs. (c) Single channel (K15-488) image showing labelling of HF bulge. (d) Low magnification image of epidermis emphasizing patterned arrangement of HF triplets (one triplet per diamond marked with dashed lines). (e) MP ontology tree. MP terms in green were used to annotate skin phenotypes in this study. (f) Schema shows how resources generated from this study can be further utilized. Scale bars, 100 μm (b,c); 200 μm (d).
