Figure 4: Krt76−/− phenotype.
From: Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen
(a) Flaky tail skin (arrow). (b) Darkly pigmented forepaws (arrows). (c) lacZ reporter expression in footpad (downloaded from Sanger Mouse Portal). (d) Stack bars showing proportion of Krt76−/− mice with abnormalities observed in epidermal wholemounts or dorsal coat colour. HET, heterozygous; HOM, homozygous; control, WT mice run each week through the Sanger MGP to ensure that all testing conditions are normal; baseline, all controls were run for a particular background, pipeline and diet combination. (e) Confocal epidermal wholemount images of WT and Krt76−/− mice. (f) Histological sections stained with haematoxylin and eosin (upper panel) and Ki67 (lower panel). Ectopic sebocytes in the IFE (arrowhead) are shown at higher magnification in the insert. Brackets indicate IFE thickness. (g) Immunostaining for the SG differentiation marker, FASN. Ectopic sebocytes are marked by arrowheads. (h) Immunostaining for the markers indicated. Thickness of each layer was quantified using Fiji (Image J) software. Stack bars show results of unpaired t-test. Error bars denote mean (n=3) with s.d., ***P<0.0002, ****P<0.0001. (i) STRING network image shows possible interaction partners of Krt76. Scale bars, 50 μm (upper panel) and 100 μm (lower panel) (e); 200 μm (f,g); 100 μm (h).
