Figure 1: Bhlhe40-deficient mice are protected from EAE.

(a) Mean clinical scores of EAE in immunized WT (n=18) or Bhlhe40−/− mice (n=18). Data are combined from four independent experiments. Incidence of disease: WT mice 17/18, Bhlhe40−/− mice 2/18. (b) H&E staining of spinal cord sections from WT and Bhlhe40−/− mice at day 13 after EAE induction. Scale bars: 200 μm in left images, 50 μm in insets. (c) Mean clinical scores of EAE in BM chimeric mice 13 weeks after bone marrow reconstitution (n=5 per group). (d) Mean clinical scores of EAE in Rag1−/− mice that received CD4+ T cells from either WT or Bhlhe40−/− mice 1 day before immunization (n=4 per group). For all figures throughout, error bars show mean±s.e.m.