Figure 2: Diminished neuroinflammation in Bhlhe40 −/− mice. | Nature Communications

Figure 2: Diminished neuroinflammation in Bhlhe40 −/− mice.

From: Bhlhe40 controls cytokine production by T cells and is essential for pathogenicity in autoimmune neuroinflammation

Figure 2

(a) Flow cytometry of CNS myeloid cells from WT and Bhlhe40−/− mice (n=3 per group) on day 16 after immunization. (b) Percentage of MHC class II+ microglia and infiltrating myeloid cells. (c–e) Flow cytometry of CD4+ T cells in the CNS of WT and Bhlhe40−/− mice (n=3 per group) on day 16 after immunization. (c) CD4+ T cell number. (d) Representative ICS for the indicated cytokines. (e) Frequency of CD4+ T cells secreting each of the indicated eight possible combinations of GM-CSF, IL-17A and IFN-γ. (f,g) Flow cytometry of γδ T cells in the CNS of WT and Bhlhe40−/− mice (n=3 per group) on day 14 after immunization. (f) Representative ICS for IFN-γ, IL-17A and GM- CSF. (g) Frequency of γδ T cells secreting IFN-γ, IL-17A or GM-CSF.

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