Figure 5: Transcriptional analysis of Bhlhe40-deficient T _H cells.

(a) Heat map of gene expression for selected T_H1, T_H2 and T_H17 lineage-specific genes in polarized WT and Bhlhe40^−/− T_H cells. (b) Pairwise comparison of WT and Bhlhe40^−/− T_H1, T_H2 and T_H17 cells. Probes that are overexpressed ≥2-fold in the indicated populations are shown in red or blue. Numbers in corners indicate the total number of probes meeting these conditions. Probes for selected relevant genes are shown in green. Coefficients of determination (R²) are indicated for each comparison. (c) Venn diagrams showing the overlap of probes differentially expressed by WT and KO T_H cell lineages. Numbers in regions indicate the number of probes with greater than or equal to a twofold difference in expression (WT>KO or WT<KO). (d) Quantitative RT-PCR analysis of Ifng, Il4 and Il17a expression in WT and Bhlhe40^−/− T_H1, T_H2 and T_H17 cells (n=3 per group). (e) Quantitative RT-PCR analysis of the expression of the indicated nine genes in WT and Bhlhe40^−/− T_H1, T_H2 and T_H17 cells (n=3 per group).