Figure 5: CARL can regulate miR-539 expression and activity. | Nature Communications

Figure 5: CARL can regulate miR-539 expression and activity.

From: CARL lncRNA inhibits anoxia-induced mitochondrial fission and apoptosis in cardiomyocytes by impairing miR-539-dependent PHB2 downregulation

Figure 5

(a) LncRNAs expression levels upon treatment with anoxia. Cardiomyocytes were untreated (control) or treated with anoxia for 24 h. LncRNAs chosen from Fantom company were analysed by qRT-PCR. Data are shown as mean±s.e.m. of four independent experiments. *P<0.05 in Student’s t-test. (b) Knockdown of CARL induces the increase of miR-539 expression levels. Cardiomyocytes were infected with adenoviral CARL-siRNA (CARL-siRNA) and its scramble form (CARL-sc). miR-539 levels were analysed by northern blot (upper panel) and CARL levels were analysed by real-time PCR (low panel). Data are shown as mean±s.e.m. of three independent experiments. *P<0.05 versus control in one-way analysis of variance. (c) Knockdown of CARL induces miR-539 activity. Cardiomyocytes were infected with adenoviral CARL-siRNA and its scramble form, then transfected with miR-539 sensor. Luciferase activity was analysed. Data are shown as mean±s.e.m. of three independent experiments. *P<0.05 versus miR-539 sensor alone in one-way analysis of variance. (d) Enforced expression of CARL reduces the expression levels of miR-539. Cardiomyocytes were infected with adenoviral CARL or β-gal. miR-539 levels were analysed by northern blot (upper panel) and CARL levels were analysed by real-time PCR (low panel). Data are shown as mean±s.e.m. of three independent experiments. *P<0.05 versus control in one-way analysis of variance. (e) CARL reduces miR-539 activity. Cardiomyocytes were infected with adenoviral CARL or β-gal, then transfected with miR-539 sensor. Luciferase activity was analysed. Data are shown as mean±s.e.m. of three independent experiments. *P<0.05 versus miR-539 sensor alone in one-way analysis of variance. (f) CARL acts as a sponge for miR-539. Cardiomyocytes were infected with adenoviral miR-539, CARL or β-gal, then transfected with miR-539 sensor. Luciferase activity was analysed. Data are shown as mean±s.e.m. of three independent experiments. *P<0.05 in one-way analysis of variance.

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