Table 2 MiHAs detected in both subjects and coded by loci harbouring ns-SNPs.

MiHA name	Detected MiHA sequence	Gene symbol	HLA allele	IC ₅₀ (nM)	aa sub.1	aa sub.2	Alternative MiHA variant	IC ₅₀ (nM)	IC ₅₀ ratio	dbSNP
(A)
MCPH1-1^R	EEINLQRNI	MCPH1	B*44:03	503	RR	RI	EEINLQINI	212	0.4	rs2083914
MDM1-1^I	VIQERVHSL	MDM1	B*08:01	61	IT	II	VTQERVHSL	401	6.6	rs962976
FAM82B-1^K	VMGNPGTFK	FAM82B	A*03:01	23	KN	KK	VMGNPGTFN	15,374	668	rs6980476
(B)
TMEM132A-1^A	AAADRVGPAA	TMEM132A	C*16:01	1,236	AP	AP	AAADRVGPPA	1,203	1	—
MAGEF1-1^A	ALAAKALAR	MAGEF1	A*03:01	136	AS	AS	ALAAKSLAR	109	0.8	rs10937187
TRIP11-1^K	DVQKKLMSL	TRIP11	B*08:01	216	KN	KN	DVQNKLMSL	534	2.5	rs145868557
IMMT-1^S	KQSASQLQK	IMMT	A*03:01	65	SP	SP	KQPASQLQK	421	6.5	rs1050301
DLGAP5-1^H	KTYHVTPMTPR	DLGAP5	A*03:01	27	HQ	HQ	KTYQVTPMTPR	48	1.8	rs8010791
ZWINT-1^R	QELDGVFQKL	ZWINT	B*44:03	366	RG	RG	QELDRVFQKL*	197	0.5	rs2241666
MIIP-1^K	SEESAVPKRSW	MIIP	B*44:03	235	KE	KE	SEESAVPERSW	245	1.0	rs2295283

For some MiHAs, one subject was homozygous and one subject heterozygous at the MiHA locus (A), whereas for other MiHAs both subjects were heterozygous at the MiHA locus (B). Selected features of the MiHAs are shown: the detected amino-acid sequence (polymorphic residues are highlighted in bold underlined), the source gene, the HLA molecule for which the MiHA has the best predicted binding affinity (IC₅₀), the translated genotype of the polymorphic loci shown in amino acids (aa) for each subject, the alternative MiHA variant and its predicted HLA-binding affinity (IC₅₀), the differential predicted HLA-binding affinity of the variant relative to the detected sequence (IC₅₀ ratio) and the dbSNP identification when the ns-SNP corresponds to a known SNP. IC₅₀ values of the alternative MiHA variants and IC₅₀ ratios are shown in italics when they show a fold difference ≥2 relative to the detected MiHAs. Further features can be found in Supplementary Data 3.
^*The alternative MiHA variant was detected by MS.

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