Figure 2: Gene discovery strategy.
(a,b) Complete-linkage hierarchical clustering of early MIA pathway gene expression in C. roseus based on our data (a) or the Medicinal Plant Genomics Resource consortium (http://medicinalplantgenomics.msu.edu) (b). Colours indicate transcriptional activation (blue) or repression (yellow) relative to untreated samples. Tissues: Fl, flower; mL, mature leaves; iL, immature leaves; St, stem; Ro, root; Sdlg, seedling. Suspension cells (CellSus): Wt, wild-type; O2, ORCA2; O3, ORCA3. Hairy roots (HairRt): Wt, wild-type; Td, TDCi; RebH, RebH_F. Treatments: Not, no treatment; MeJA, methyl jasmonate (6, 12 or 24 h); Con, mock; YE, yeast extract. (c) Candidate P450 protein hits in the epidermis and mesophyll samples from proteomics analysis±s.d. (n=3). GES, geraniol synthase; G8O, geraniol 8-oxidase; 8-HGO, 8-hydroxygeraniol oxidoreductase; IS, iridoid synthase; IO, iridoid oxidase; 7-DLGT, 7-deoxyloganetic acid glucosyl transferase; 7-DLH, 7-deoxyloganic acid hydroxylase; LAMT, loganic acid O-methyltransferase; SGD, strictosidine β-D-glucosidase; SLS, secologanin synthase; STR, strictosidine synthase (1–3: three related genes); TDC, tryptophan decarboxylase.
