Figure 1: Analysis of the lar gene cluster and its encoded Lar proteins.
From: Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system
(a) lar locus of L. plantarum with the two operons larR(MN)QO and larA–E. (b) Effect of larA–E and larQO deletions in L. plantarum on specific Lar activity in crude extracts. Supplementation assays of the ΔlarQO mutant with CoCl2 (1 mM) and NiCl2 (0.2, 0.4, 0.8 and 1.5 mM). (c) Specific Lar activity in crude extracts after the expression of lar genes in Lc. lactis: larA–E operon ±NiCl2 (1 mM), in frame deletions of individual genes (Δ) in the larA–E operon with NiCl2 supplementation (1 mM). Data in b and c are average of quadruplicates from one representative experiment of three independent experiments showing similar results. The error bars represent the 95% confidence interval (Student’s t-test).
