Figure 5: XANES and EXAFS spectra.
From: Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system
(a) Ni K-edge XANES spectra of LarATt and LarALp showing a four-square planar or five-square pyramidal coordination geometry for nickel binding; 10 mM Tris buffer, pH 7.5, 20% glycerol. (b) Ni K-edge EXAFS spectra of LarATt and fit for N2H1H1S1. Fourier-transformed EXAFS spectra (no phase correction, FT window=2–12.5 Å−1). Inset: k3-weighted unfiltered EXAFS spectra; data (circles), best fit (line). (c) Ni K-edge EXAFS spectra of LarATt and fit for GH2H1S1. Fourier-transformed EXAFS spectra (no phase correction, FT window=2–12.5 Å−1). Inset: k3-weighted unfiltered EXAFS spectra; data (circles), best fit (line). N: N/O scatterers, H: histidine scatterer, S: S/Cl scatterers and G: glycerol scatterer.
