Figure 3: Enhanced T-cell-mediated immune responses in JKAP-knockout mice.
From: The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck
(a) ELISAs of serum IL-2, IFN-γ, IL-4 and IL-17 levels in unimmunized (un) or at day 7 in KLH-immunized (KLH) JKAP-knockout mice and their wild-type littermates in B6 background (F5). WT, n=4; KO, n=4. (b,c) ELISAs (b) and [3H]thymidine incorporation assay (c) of T cells from draining lymph nodes of KLH-immunized mice at day 7 on restimulation with KLH for 48 and 72 h, respectively. (d,e) ELISAs of nitrophenol-KLH-specific antibody (NP-specific antibody) production in the sera of mice at 14 days after primary immunization (d) or 7 days after secondary immunization (e); results are presented relative to those of the serum from a KLH-immunized wild-type mouse. Data are presented as means±s.d. Two-tailed Student’s t-test, *P<0.05. Data are representative of three independent experiments. WT, wild-type; KO, JKAP-knockout.
