Figure 3: Wnt signalling is associated with the development of the preorbital region of the zebrafish skull. | Nature Communications

Figure 3: Wnt signalling is associated with the development of the preorbital region of the zebrafish skull.

From: Wnt signalling underlies the evolution of new phenotypes and craniofacial variability in Lake Malawi cichlids

Figure 3

(a,b) At 48 hours post fertilization (hpf) the Wnt report line, Tg(7xTCF-Xla.Siam:GFP), shows a clear and distinct output in Wnt signalling in the preorbital (po) domain of the zebrafish skull. (c) Tg zebrafish treated with LiCl exhibit a marked expansion of GFP expression in the po region of the skull, which suggests that LiCl treatment has a direct effect on Wnt signalling in this anatomical region. (d-e) Zebrafish treated with 10 mM of LiCl at 48hpf, show a marked reduction in preorbital outgrowth at 120 hpf. Asterisks in (e) indicates an apparent absence of the preorbital region of the skull. Scale bars, 200 μm.

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