Figure 4: MHC-I induction in VM DA neurons is dependent on oxidative stress.
From: MHC-I expression renders catecholaminergic neurons susceptible to T-cell-mediated degeneration
(a) Example of TH/MHC-I double immunolabel of primary cultures of VM neurons after treatment with L-DOPA, which induced the presence of both NM (arrow) and MHC-I. Scale bar, 30 μm. (b) Fraction of TH+ and TH− neurons that displayed NM following L-DOPA. Data are presented as mean±s.e.m. (NS, two-tailed Student’s t-test). (c) The fraction of TH+ and TH− neurons that displayed plasma membrane MHC-I following L-DOPA. Data are presented as mean±s.e.m. (*P<0.05, two-tailed Student’s t-test). Each experiment was repeated at least in triplicate and within each experiment, each condition was also performed at least three times. For each condition, neurons on n=24 fields at × 20 were quantified. BF, brightfield; CTRL, control; NS, nonsignificant.
