Figure 6: VM DA neurons that display antigen are killed by CD8+ T cells.
From: MHC-I expression renders catecholaminergic neurons susceptible to T-cell-mediated degeneration
(a) The fraction of VM DA neurons surviving in the presence of SIINFEKL, OT-1 cells pre-pulsed with SIINFEKL, and IFN-γ (100 ng ml−1) in cultures obtained from wild-type and β2m KO mice. Data are presented as the mean±s.e.m. (NS; **P<0.01, One-way analysis of variance (ANOVA) with Tukey post hoc test). (b) Survival of VM DA neurons incubated with IFN-γ, SIINFEKL peptide and OT-1 cells with the pan-caspase inhibitor Z-VAD-FMK, the Fas/Fas ligand antagonist, Kp7-6, the perforin/granzyme antagonist, concanamycin A or the combination of Kp7-6 and concanamycin A. Data are presented as the mean±s.e.m. (NS; *P<0.05, ***P<0.001, one-way ANOVA with Tukey post hoc test). (c) VM DA neuron survival with SIINFEKL, OT-1 cells pre-pulsed with SIINFEKL and microglial medium previously exposed to LPS, α-syn or NM. Data are presented as the mean±s.e.m. (NS; *P<0.05, **P<0.01; ***P<0.001, one-way ANOVA with Tukey post hoc test). Each experiment was repeated at least in triplicate and within each experiment, each condition was also performed at least three times. For each condition, neurons on n=24 fields at × 20 were quantified. ConA, concanamycin A; CTRL, control; IFN, interferon gamma; NS, nonsignificant; SIIN, SIINFEKL; Veh, vehicle.
