Table 1 Summary of peptides studied for the assembly.

From: Tyrosine-mediated two-dimensional peptide assembly and its role as a bio-inspired catalytic scaffold

Facet-forming peptides

Non-facet-forming peptides

Sequence

Kinetics of facet formation*

Sequence

Observed structures †

YYCYY

Fast

YCY

Random aggregates

YYYCYYY

Very fast‡

CYY

Nanofibre

YYACAYY

Very fast

YY

Nanofibre

YYAACAAYY

Very fast

YYY

Nanofibre

YYGCGYY

Very slow

YYYY

Random aggregates

YFCFY

Fast

YYAYY

Planar structure

FYCYF

Slow

CYYCYYC

Random aggregates

FFCFF

Very slow

YYAKAYY

Planar structure

  

YYAEAYY

Planar structure

  1. The peptides were dissolved in 50 mM HEPES buffer (pH 7.4) for a final concentration of 1.1 mM. A total of 80 μl of peptide solution was dropped onto siliconized glass, and the facet formation of each droplet was monitored.
  2. *We defined the facet formation time as the point when the diameter of a facet reaches 2 mm. The kinetics of facet formation was described by the following criteria: ‘Very fast’: formation within 1 min; ‘Fast’: between 1 and 30 min; ‘Slow’: between 30 and 60 min; ‘Very slow’: more than 60 min, small facet.
  3. †For the peptide sequences that cannot drive facet formation, the assembled peptide structures were analysed by TEM.
  4. ‡Wrinkled multiple facets (Supplementary Fig. 22).
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