Figure 5: Chondrocytes of reserve zone are recruited into proliferation in Gsα cKO mice. | Nature Communications

Figure 5: Chondrocytes of reserve zone are recruited into proliferation in Gsα cKO mice.

From: G-protein stimulatory subunit alpha and Gq/11α G-proteins are both required to maintain quiescent stem-like chondrocytes

Figure 5

(a) Protocol for BrdU labelling of reserve zone cells (stem-like chondrocytes). (b) Representative images of the growth plates of control (Gsα fl/fl) and Gsα cKO (Col2-CreERt; Gsα fl/fl) mice shown in a,c. Sections are stained with H&E. (c) BrdU-detection revealed BrdU-positive stem-like chondrocytes in control, but not in Gsα cKO growth plates. Sections were counterstained with eosin (bone, pink) and Alcian Blue (cartilage, blue). (d) Modified protocol for BrdU labelling of stem-like chondrocytes aiming to detect stem-like cell proliferation. Mice were labelled with BrdU exactly as in a, but were killed earlier and were labelled with EdU 4 and 20 h before death. (e) Sections from control (Gsα fl/fl) and Gsα cKO (Col2-CreERt; Gsα fl/fl) mice treated as shown in d were stained with both BrdU and EdU. Double BrdU- and EdU-labelled cells (arrows) were observed in Gsα cKO mice but almost never in control mice. ep=epiphyseal bone. Scale bars, 100 μm.

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